f1000research-6-13732-g0002

Real-time quaking-induced conversion (RT-QuIC) responses from reactions seeded with cerebrospinal fluid (CSF) from two sporadic Creutzfeldt-Jakob disease (sCJD) cases and a positive sCJD CSF control: The RT-QuIC from an unseeded reaction and a reaction seeded with brain homogenate from Alzheimer’s disease are also shown. | Neil McKenzie, University of Edinburgh, Edinburgh, UK. AD BH, Alzheimer’s disease brain homogenate; RFU, relative fluorescence units; ThT, thioflavin T.