Kleihauer–Betke “KB” test/stain, or Acid elution test, is a blood test used to measure the amount of fetal haemoglobin transferred from a fetus to a mother’s bloodstream.
- Standard method of quantitating fetal-maternal haemorrhage (FMH)
- Performed on Rh-negative mothers to determine the required dose of Rho(D) immune globulin (RhIg) to inhibit formation of Rh-antibodies in the mother and prevent Rh disease in future Rh-positive children.
History
Enno Kleihauer and Klaus Betke described it in 1957.
Steps
- Peripheral blood smear is made from the maternal postpartum sample and treated with acid.
- Fetal cells remain intact because of high concentrations of HbF, while HbA is eluted from the maternal cells.
- After acid treatment the slides are washed, stained, and examined microscopically.
- The number of fetal cells (which take up the stain) are counted per number of maternal cells (which appear as ghost cells) to give % fetal cells.
- The volume of fetal bleed is then calculated to determine how much additional RhIG is required.